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Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.
Antibody dilution
1:122
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA045761
Antibody HPA053721
Standard validation
Supported
Figure description
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells and strong nuclear staining i non-germinal center cells.
Expression
RNA: detected in 37 tissues Protein: detected in 59 cell types
Retrieval
HIER pH6
Antibody dilution
1:2500
Literature conformity
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA045761
Antibody HPA053721
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
32.2, 24.1, 19.5
32.2
Antibody dilution
1:2190
1:610
PROTEIN ARRAY
Antibody HPA045761
Antibody HPA053721
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
