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ANTIBODY INFORMATION
 
 

Antibody HPA000842

  

Antibody HPA001030

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA000842   HPA001030  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 13   13  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA000842

  

Antibody HPA001030

  
Immunocytochemistry

Approved   Approved  
Western blot

Uncertain    
Protein array

Approved   Approved  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000842

  

Antibody HPA001030

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.  

Antibody dilution

 1:95   1:35  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

WESTERN BLOT
 
 

Antibody HPA000842

  

Antibody HPA001030

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		    

   

Figure description

    

Target mass (kDa)

 83.2, 25.8    

Antibody dilution

 1:250    

PROTEIN ARRAY
 
 

Antibody HPA000842

  

Antibody HPA001030

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1000   1:2000  

ANTIGEN INFORMATION
 
 

Antibody HPA000842

  

Antibody HPA001030

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 130   127  

Antigen sequence

 
KRANSYEDAMMPFPGATIISQLLKNNMNKNGGTEPSFQASGLSSTGSEVH
QEDICSNSSRDSPPECLSPFGRPTMSQFDVDRLCDEHLRAKRARVENIIR
GMSHSPSVALRGNENEREMAPQSVSPRESY
  
TAMSQVVDTVVKVFSAKPSRQVPQVFPPLQIPQARFAVNGENHNFHTANQ
RLQCFGDVIIPNPLDTFGSVQMPSSTDQTEALPLVVRKNSSEQSASGPAT
GGHHQPLHQSPLSATAGFTTPSFRHPF
 

Matching transcripts

 PROX1-001 - ENSP00000355925 [99%]
PROX1-002 - ENSP00000400694 [99%]
PROX1-004 - ENSP00000419517 [99%]
PROX1-201 - ENSP00000261454 [99%]
PROX1-202 - ENSP00000420283 [99%]
  PROX1-001 - ENSP00000355925 [94%]
PROX1-002 - ENSP00000400694 [94%]
PROX1-201 - ENSP00000261454 [94%]
PROX1-202 - ENSP00000420283 [94%]
 

Other gene match

    
ANTIGEN VIEW
PROX1-001
PROX1-002
PROX1-004
PROX1-201
PROX1-202
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.