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Immunohistochemical staining of human testis shows strong cytoplasmic positivity in spermatids and spermatozoa.
Immunohistochemical staining of human testis shows strong cytoplasmic positivity in subset of cells in seminiferus ducts.
Expression
RNA: detected in 1 tissues Protein: detected in 4 cell types
RNA: detected in 1 tissues Protein: detected in 16 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:250
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA026744
Antibody HPA043297
Standard validation
Uncertain
Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Current setup is not applicable due to low RNA count.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY410624)
Target mass (kDa)
32.1
32.1
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA026744
Antibody HPA043297
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
