RNF146 - Antibodies - The Human Protein Atlas

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RNF146

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA027158

Antibody HPA027209

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA027158

HPA027209

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA027158

Antibody HPA027209

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA027158

Antibody HPA027209

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.

Antibody dilution

1:200

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA027158

Antibody HPA027209

Standard validation

Approved

Figure description

Immunohistochemical staining of human placenta shows moderate cytoplasmic and nuclear positivity in decidual cells.

Immunohistochemical staining of human placenta shows strong nuclear positivity in decidual cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 61 cell types

RNA: detected in 37 tissues
Protein: detected in 56 cell types

Retrieval

HIER pH6

Antibody dilution

1:20

1:400

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA027158

Antibody HPA027209

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

38.9, 38.8

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA027158

Antibody HPA027209

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3000

ANTIGEN INFORMATION

Antibody HPA027158

Antibody HPA027209

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

IPEDFLDKPTLLSPEELKAASRGNGEYAWYYEGRNGWWQYDERTSRELED
AFSKGKKNTEMLIAGFLYVADLEN

MVQYRRNEHGRRRKIKRDIIDIPKKGVAGLRLDCDANTVNLARESSADGA
DSVSAQSGASVQPLVSSVRPLTSVDGQLTSPATPSPD

Matching transcripts

RNF146-001 - ENSP00000357297 [100%]
RNF146-008 - ENSP00000476814 [100%]
RNF146-011 - ENSP00000477168 [100%]
RNF146-201 - ENSP00000309365 [100%]
RNF146-202 - ENSP00000479890 [100%]

Other gene match

ANTIGEN VIEW

RNF146-001	RNF146-008	RNF146-011	RNF146-201	RNF146-202

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.