We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.
Antibody staining overlaps with antibody HPA031075.
Validated
Antibody staining overlaps with antibody HPA031074.
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
Antibody dilution
1:20
1:10
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA031074
Antibody HPA031075
Antibody CAB005123
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human stomach, upper shows moderate positivity in plasma and stromal cells.
Immunohistochemical staining of human smooth muscle shows cytoplasmic and nuclear positivity in smooth muscle cells.
Expression
RNA: detected in 37 tissues Protein: detected in 52 cell types
RNA: detected in 37 tissues Protein: detected in 36 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:10
1:100
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA031074
Antibody HPA031075
Antibody CAB005123
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419668)
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
38.1
38.1
38.1
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA031074
Antibody HPA031075
Antibody CAB005123
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
