UBE2B - Antibodies - The Human Protein Atlas

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UBE2B

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA003875

Antibody HPA051765

Antibody HPA065898

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA003875

HPA051765

HPA065898

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

UBE2A - ENSG00000077721 [96%]

UBE2A - ENSG00000077721 [100%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA003875

Antibody HPA051765

Antibody HPA065898

Immunocytochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA003875

Antibody HPA051765

Antibody HPA065898

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm & cytosol.

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.

Immunofluorescent staining of human cell line A-431 shows localization to nucleus & plasma membrane.

Antibody dilution

1:47

1:200

1:4

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

WESTERN BLOT

Antibody HPA003875

Antibody HPA051765

Antibody HPA065898

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

17.3, 17, 16.1, 15.4, 14

17.3, 17, 16.1, 13.8, 13.6, 8.8

17.3, 17, 16.1, 15.4, 14, 13.8

Antibody dilution

1:250

1:1000

1:80

PROTEIN ARRAY

Antibody HPA003875

Antibody HPA051765

Antibody HPA065898

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:8000

1:500

ANTIGEN INFORMATION

Antibody HPA003875

Antibody HPA051765

Antibody HPA065898

Antigen

Recombinant protein fragment

Length (aa)

120

Antigen sequence

LMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIE
FSEEYPNKPPTVRFLSKMFHPNVYADGSICLDILQNRWSPTYDVSSILTS
IQSLLDEPNPNSPANSQAAQ

IQSLLDEPNPNSPANSQAAQLYQENKREYEKRVSAIVEQSW

APSENNIMVWNAVIFGPEGTPFEDGTFK

Matching transcripts

UBE2B-001 - ENSP00000265339 [100%]
UBE2B-008 - ENSP00000425137 [93%]
UBE2B-005 - ENSP00000426364 [86%]

UBE2B-001 - ENSP00000265339 [100%]
UBE2B-008 - ENSP00000425137 [100%]

UBE2B-001 - ENSP00000265339 [96%]
UBE2B-005 - ENSP00000426364 [96%]
UBE2B-008 - ENSP00000425137 [96%]

Other gene match

UBE2A - ENSG00000077721 [96%]

UBE2A - ENSG00000077721 [100%]

ANTIGEN VIEW

UBE2B-001	UBE2B-005	UBE2B-008

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.