ELF1 - Antibodies - The Human Protein Atlas

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ELF1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA001755

Antibody CAB005172

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA001755

sc-631

Host species

Rabbit

Clonality

pAb

msAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Affinity

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA001755

Antibody CAB005172

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA001755

Antibody CAB005172

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

Antibody dilution

1:125

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA001755

Antibody CAB005172

Standard validation

Approved

Figure description

Immunohistochemical staining of human lymph node shows distinct nuclear positivity in germinal center cells.

Immunohistochemical staining of human lymph node shows distinct nuclear positivity in all cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 53 cell types

RNA: detected in 37 tissues
Protein: detected in 67 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:500

Literature conformity

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA001755

Antibody CAB005172

Standard validation

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

67.5, 67.1, 64.9, 39

67.5, 67.1, 64.9, 39, 2.8

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA001755

Antibody CAB005172

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA001755

Antibody CAB005172

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

135

Antigen sequence

PSSSIESSDPSLSSSATSNRNQTSRSRVSSSPGVKGGATTVLKPGNSKAA
KPKDPVEVAQPSEVLRTVQPTQSPYPTQLFRTVHVVQPVQAVPEGEAART
STMQDETLNSSVQSIRTIQAPTQVPVVVSPRNQQL

Matching transcripts

ELF1-003 - ENSP00000239882 [100%]
ELF1-004 - ENSP00000486912 [100%]
ELF1-005 - ENSP00000489586 [100%]
ELF1-201 - ENSP00000405580 [100%]

Other gene match

ANTIGEN VIEW

ELF1-003	ELF1-004	ELF1-005	ELF1-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.