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EPHX2

ANTIBODY INFORMATION
 
 

Antibody HPA023094

  

Antibody HPA023660

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA023094   HPA023660  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 5   5  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA023094

  

Antibody HPA023660

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved   Approved  
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA023094

  

Antibody HPA023660

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA023660.		  
Validated

Antibody staining overlaps with antibody HPA023094.		  
 

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

 1:50   1:57  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA023094

  

Antibody HPA023660

  

Standard validation

 Approved   Approved  

   

Figure description

 Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal centers.   Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal centers.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 58 cell types		   RNA: detected in 37 tissues
Protein: detected in 40 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:150  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA023094

  

Antibody HPA023660

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 62.6, 58.9, 57.1, 55.6, 42.1   62.6, 57.1, 55.6  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA023094

  

Antibody HPA023660

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA023094

  

Antibody HPA023660

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 82   81  

Antigen sequence

 
YRNMERNWKWACKSLGRKILIPALMVTAEKDFVLVPQMSQHMEDWIPHLK
RGHIEDCGHWTQMDKPTEVNQILIKWLDSDAR
  
NLKPARDLGMVTILVQDTDTALKELEKVTGIQLLNTPAPLPTSCNPSDMS
HGYVTVKPRVRLHFVELGSGPAVCLCHGFPE
 

Matching transcripts

 EPHX2-001 - ENSP00000430269 [100%]
EPHX2-002 - ENSP00000369843 [100%]
EPHX2-003 - ENSP00000428875 [100%]
EPHX2-005 - ENSP00000427956 [100%]
EPHX2-008 - ENSP00000430302 [100%]
  EPHX2-001 - ENSP00000430269 [100%]
EPHX2-002 - ENSP00000369843 [100%]
EPHX2-008 - ENSP00000430302 [100%]
 

Other gene match

    
ANTIGEN VIEW
EPHX2-001
EPHX2-002
EPHX2-003
EPHX2-005
EPHX2-008
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.