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Immunofluorescent staining of human cell line CACO-2 shows localization to mitochondria.
Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.
Antibody dilution
1:97
1:125
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA008620
Antibody HPA008691
Antibody CAB019286
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human heart muscle shows strong cytoplasmic positivity in myocytes.
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 78 cell types
RNA: detected in 37 tissues Protein: detected in 76 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:200
1:500
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008620
Antibody HPA008691
Antibody CAB019286
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY418643)