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CEP89

ANTIBODY INFORMATION
 
 

Antibody HPA039382

  

Antibody HPA040056

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA039382   HPA040056  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 12   7  

References

   1  

VALIDATION SUMMARY
 
 

Antibody HPA039382

  

Antibody HPA040056

  
Immunocytochemistry

Supported   Supported  
Immunohistochemistry

Uncertain   Uncertain  
Western blot

Uncertain   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA039382

  

Antibody HPA040056

  

Standard validation

 Supported   Supported  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.  

Antibody dilution

 1:200   1:154  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA039382

  

Antibody HPA040056

  

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunohistochemical staining of human lateral ventricle shows strong cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in tubular cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 55 cell types		   RNA: detected in 36 tissues
Protein: detected in 15 cell types		  

Retrieval

 HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:700  

Literature conformity

 No avaliable gene/protein characterization data.   No avaliable gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA039382

  

Antibody HPA040056

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 89.6   89.6  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA039382

  

Antibody HPA040056

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:6000  

ANTIGEN INFORMATION
 
 

Antibody HPA039382

  

Antibody HPA040056

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 81   116  

Antigen sequence

 
QVLKDKQEVLDQALQQNREMEGELEVIWESTFRENRRIRELLQDTLTRTG
VQDNPRALVAPSLNGVSQADLLDGCDVCSYD
  
KINRKSQKKIEVLKKQVEKAMGNEMSAHQYLANLVGLAENITQERDSLMC
LAKCLESEKDGVLNKVIKSNIRLGKLEEKVKGYKKQAALKLGDISHRLLE
QQEDFAGKTAQYRQEM
 

Matching transcripts

 CEP89-001 - ENSP00000306105 [100%]
  CEP89-001 - ENSP00000306105 [100%]
 

Other gene match

    
ANTIGEN VIEW
CEP89-001

RELEVANT PUBLICATIONS
 
 

Antibody HPA039382

  

Antibody HPA040056

  

1.

   The snRNA-processing complex, Integrator, is required for ciliogenesis and dynein recruitment to the nuclear envelope via distinct mechanisms
Jodoin JN et al
Biol Open 2013;2(12):1390-6
  
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.