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Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & mitochondria.
Antibody dilution
1:40
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA018842
Antibody HPA019076
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human gallbladder shows moderate cytoplasmic positivity in glandular cells.
Immunohistochemical staining of human urinary bladder shows moderate positivity in urothelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 38 cell types
RNA: detected in 37 tissues Protein: detected in 34 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:300
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
Standard validation
Approved
Figure description
Strong labelling of nuclei of ependymal cells and subsets of neurons. Weak labelling of soma and dendrites.
WESTERN BLOT
Antibody HPA018842
Antibody HPA019076
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
41.9, 37.1, 36.1, 27.5
41.9, 37.1, 36.1
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA018842
Antibody HPA019076
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
