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CALD1

ANTIBODY INFORMATION
 
 

Antibody HPA008066

  

Antibody HPA017330

  

Antibody CAB000006

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   DakoCytomation
  

Product name

 HPA008066   HPA017330   M3557  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Supernatant  

Other gene match

      

Released in version

 3   4   1  

References

 4   3    

VALIDATION SUMMARY
 
 

Antibody HPA008066

  

Antibody HPA017330

  

Antibody CAB000006

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA008066

  

Antibody HPA017330

  

Antibody CAB000006

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA017330.		  
Validated

Antibody staining overlaps with antibody HPA008066.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.   Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane & actin filaments.    

Antibody dilution

 1:20   1:333    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA008066

  

Antibody HPA017330

  

Antibody CAB000006

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		    

 

 

  
 

 

    

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 42 cell lines.    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.   Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity.   Immunohistochemical staining of human smooth muscle shows strong cytoplasmic positivity in smooth muscle cells.  

Expression

 RNA: detected in 36 tissues
Protein: detected in 31 cell types		   RNA: detected in 36 tissues
Protein: detected in 56 cell types		   RNA: detected in 36 tissues
Protein: detected in 5 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH9  

Antibody dilution

 1:100   1:2000   1:200  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA008066

  

Antibody HPA017330

  

Antibody CAB000006

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 93.2, 65.6, 64.3, 64.2, 62.7, 61.2   93.2, 65.6, 64.3, 64.2, 62.7, 61.2, 54.2   93.2, 65.6, 64.3, 64.2, 62.7, 61.2, 54.2, 16, 9  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA008066

  

Antibody HPA017330

  

Antibody CAB000006

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:500   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA008066

  

Antibody HPA017330

  

Antibody CAB000006

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein  

Length (aa)

 147   132    

Antigen sequence

 
SLKIEERAEFLNKSVQKSSGVKSTHQAAIVSKIDSRLEQYTSAIEGTKSA
KPTKPAASDLPVPAEGVRNIKSMWEKGNVFSSPTAAGTPNKETAGLKVGV
SSRINEWLTKTPDGNKSPAPKPSDLRPGDVSSKRNLWEKQSVDKVTS
  
MTHKLKHTENTFSRPGGRASVDTKEAEGAPQVEAGKRLEELRRRRGETES
EEFEKLKQKQQEAALELEELKKKREERRKVLEEEEQRRKQEEADRKLREE
EEKRRLKEEIERRRAEAAEKRQKMPEDGLSDD
  

											 
																				

																		

										
											
Matching transcripts

												
																					CALD1-001 - ENSP00000354513 [100%] 
CALD1-002 - ENSP00000398826 [100%] 
CALD1-005 - ENSP00000354826 [100%] 
CALD1-010 - ENSP00000393621 [100%] 
CALD1-201 - ENSP00000376826 [100%] 
CALD1-003 - ENSP00000395710 [99%] 
CALD1-029 - ENSP00000419673 [99%] 

											 
																						CALD1-001 - ENSP00000354513 [100%] 
CALD1-002 - ENSP00000398826 [100%] 
CALD1-003 - ENSP00000395710 [100%] 
CALD1-005 - ENSP00000354826 [100%] 
CALD1-010 - ENSP00000393621 [100%] 
CALD1-012 - ENSP00000411476 [100%] 
CALD1-029 - ENSP00000419673 [100%] 
CALD1-201 - ENSP00000376826 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
CALD1-001
CALD1-002
CALD1-003
CALD1-005
CALD1-010
CALD1-012
CALD1-029
CALD1-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA008066

												
										 
																				
											
											
											
Antibody HPA017330

												
										 
																				
											
											
											
Antibody CAB000006

												
										 
																		

																	

										
											
1.

												
																						Mapping the subcellular protein distribution in three human cell lines

	Fagerberg L et al

	J Proteome Res 2011;10(8):3766-77

			
											 
																							Histochemical localization of caldesmon in the CNS and ganglia of the mouse

	Köhler CN et al

	J Histochem Cytochem 2011;59(5):504-17

			
											 
																						
											 
																				

																		

										
											
2.

												
																						Histochemical localization of caldesmon in the CNS and ganglia of the mouse

	Köhler CN et al

	J Histochem Cytochem 2011;59(5):504-17

			
											 
																							Profiling post-centrifugation delay of serum and plasma with antibody bead arrays

	Qundos U et al

	J Proteomics 2013;95:46-54

			
											 
																						
											 
																				

																		

										
											
3.

												
																						Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

	Stadler C et al

	J Proteomics 2012;75(7):2236-51

			
											 
																							Antibodies biotinylated using a synthetic Z-domain from protein A provide stringent in situ protein detection

	Andersson S et al

	J Histochem Cytochem 2013;61(11):773-84

			
											 
																						
											 
																				

																		

										
											
4.

												
																						Stromal gene expression defines poor-prognosis subtypes in colorectal cancer

	Calon A et al

	Nat Genet 2015;47(4):320-9

			Application: IHC				
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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