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VPS26A

ANTIBODY INFORMATION
 
 

Antibody HPA057498

  

Antibody CAB011602

  

Antibody CAB075733

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Abcam plc
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA057498   ab23892   AMAb90967  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   msAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity   Protein A/G  

Other gene match

      

Released in version

 14   4   14  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA057498

  

Antibody CAB011602

  

Antibody CAB075733

  
Immunocytochemistry


Validated
   Supported    
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Approved      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA057498

  

Antibody CAB011602

  

Antibody CAB075733

  

Formal validation: Tagged protein
Validated

Antibody staining overlaps with GFP tagged protein		      

     

Figure description

 Immunofluorescent staining of transgenic HeLa cells show antibody staining in endosome & lysosome and GFP expression in endosome & lysosome.      

Antibody dilution

 1:9      
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to endosomes & lysosomes.   Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.    

Antibody dilution

 1:9   1:125    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA057498

  

Antibody CAB011602

  

Antibody CAB075733

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human fallopian tube shows cytoplasmic positivity in glandular cells.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells of seminiferus ducts.   Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells ins seminiferous ducts and Leydig cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 48 cell types		   RNA: detected in 37 tissues
Protein: detected in 71 cell types		   RNA: detected in 37 tissues
Protein: detected in 72 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:8   1:500   1:200  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA057498

  

Antibody CAB011602

  

Antibody CAB075733

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 38.2   38.2, 28.9, 26.2   38.2, 28.9, 26.2  

Antibody dilution

 1:80   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA057498

  

Antibody CAB011602

  

Antibody CAB075733

  

Standard validation

 Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		      

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:500      

ANTIGEN INFORMATION
 
 

Antibody HPA057498

  

Antibody CAB011602

  

Antibody CAB075733

  

Antigen

 Recombinant protein fragment   Synthetic peptide   Recombinant protein  

Length (aa)

 26      

Antigen sequence

 
LRKQRTNFHQRFESPESQASAEQPEM
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					VPS26A-001 - ENSP00000362480 [100%] 
VPS26A-201 - ENSP00000263559 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
VPS26A-001
VPS26A-201
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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