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Antibody staining overlaps with antibody HPA063575.
Validated
Antibody staining overlaps with antibody HPA049975.
Standard validation
Approved
Approved
Figure description
Immunofluorescent staining of human cell line HeLa shows localization to nucleoplasm.
Immunofluorescent staining of human cell line SiHa shows localization to nucleoplasm.
Antibody dilution
1:26
1:114
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA049975
Antibody HPA063575
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic and nuclear positivity in astrocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 31 cell types
Retrieval
HIER pH6
Antibody dilution
1:25
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA049975
Antibody HPA063575
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
58.5
58.5, 54.4
Antibody dilution
1:130
1:250
PROTEIN ARRAY
Antibody HPA049975
Antibody HPA063575
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
