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Immunofluorescent staining of human cell line U-2 OS shows localization to plasma membrane.
Antibody dilution
1:25
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA017737
Antibody CAB009449
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human oral mucosa shows moderate cytoplasmic positivity in superficial cells of squamous epithelia.
Immunohistochemical staining of human esophagus shows moderate membranous positivity in squamous epithelial cells.
Expression
RNA: detected in 26 tissues Protein: detected in 11 cell types
RNA: detected in 26 tissues Protein: detected in 7 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:3500
1:2000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA017737
Antibody CAB009449
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY400936)
Target mass (kDa)
12.3
12.3
Antibody dilution
1:250
1:500
PROTEIN ARRAY
Antibody HPA017737
Antibody CAB009449
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
