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MAGEA10

ANTIBODY INFORMATION
 
 

Antibody HPA003333

  

Antibody HPA070780

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA003333   HPA070780  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 9   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA003333

  

Antibody HPA070780

  
Immunocytochemistry

  Supported  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA003333

  

Antibody HPA070780

  

Standard validation

   Supported  

   

Figure description

   Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol.  

Antibody dilution

   1:135  

Literature conformity

   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA003333

  

Antibody HPA070780

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human placenta shows strong nuclear and cytoplasmic positivity in trophoblastic cells.    

Expression

 RNA: detected in 2 tissues
Protein: detected in 28 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:75    

Literature conformity

 Partly consistent with gene/protein characterization data.    

RNA consistency

 Mainly not consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA003333

  

Antibody HPA070780

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		   Uncertain

Analysis performed using a standard panel of samples.
Current setup is not applicable due to low RNA count.		  

   

Figure description

    

Target mass (kDa)

 40.8, 19.9, 17.2   40.8, 19.9, 17.2, 7.4, 6.1  

Antibody dilution

 1:250   1:450  

PROTEIN ARRAY
 
 

Antibody HPA003333

  

Antibody HPA070780

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:2000   1:5400  

ANTIGEN INFORMATION
 
 

Antibody HPA003333

  

Antibody HPA070780

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 107   44  

Antigen sequence

 
TSSSFPSSFPSSSSSSSSSCYPLIPSTPEEVSADDETPNPPQSAQIACSS
PSVVASLPLDQSDEGSSSQKEESPSTLQVLPDSESLPRSEIDEKVTDLVQ
FLLFKYQ
  
MPRAPKRQRCMPEEDLQSQSETQGLEGAQAPLAVEEDASSSTST
 

Matching transcripts

 MAGEA10-001 - ENSP00000359347 [100%]
MAGEA10-002 - ENSP00000391977 [100%]
MAGEA10-003 - ENSP00000406161 [100%]
MAGEA10-201 - ENSP00000244096 [100%]
  MAGEA10-001 - ENSP00000359347 [100%]
MAGEA10-002 - ENSP00000391977 [100%]
MAGEA10-003 - ENSP00000406161 [100%]
MAGEA10-004 - ENSP00000464160 [100%]
MAGEA10-005 - ENSP00000462833 [100%]
MAGEA10-201 - ENSP00000244096 [100%]
 

Other gene match

    
ANTIGEN VIEW
MAGEA10-001
MAGEA10-002
MAGEA10-003
MAGEA10-004
MAGEA10-005
MAGEA10-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.