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Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA012368 across 44 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA009300 across 44 cell lines.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in megakaryocytes.
Immunohistochemical staining of human spleen shows distinct positivity in endothelial cells and in a subset of red pulp cells.
Expression
RNA: detected in 37 tissues Protein: detected in 16 cell types
RNA: detected in 37 tissues Protein: detected in 11 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:10
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA009300
Antibody HPA012368
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415997)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415997)
Target mass (kDa)
68.6
68.6
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA009300
Antibody HPA012368
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
