STAT5A - Antibodies - The Human Protein Atlas

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STAT5A

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA027873

Antibody HPA042128

Antibody HPA049883

Antibody HPA051156

Antibody CAB003860

Provider

Atlas Antibodies
Sigma-Aldrich

Epitomics

Product name

HPA027873

HPA042128

HPA049883

HPA051156

1289-1

Host species

Rabbit

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Supernatant

Other gene match

STAT5B - ENSG00000173757 [100%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA027873

Antibody HPA042128

Antibody HPA049883

Antibody HPA051156

Antibody CAB003860

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA027873

Antibody HPA042128

Antibody HPA049883

Antibody HPA051156

Antibody CAB003860

Formal validation: Tagged protein

Validated

Antibody staining overlaps with GFP tagged protein

Figure description

Immunofluorescent staining of transgenic HeLa cells show antibody staining in cytoplasm & nucleus but excluded from the nucleoli and GFP expression in cytoplasm & nucleus but excluded from the nucleoli.

Antibody dilution

1:53

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

Antibody dilution

1:50

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA027873

Antibody HPA042128

Antibody HPA049883

Antibody HPA051156

Antibody CAB003860

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Standard validation

Approved

Figure description

Immunohistochemical staining of human lymph node shows distinct cytoplasmic positivity in subsets of lymphoid cells.

Immunohistochemical staining of human lymph node shows strong nuclear positivity in non-germinal center cells.

Immunohistochemical staining of human lymph node shows distinct cytoplasmic positivity in subsets of lymphoid cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 43 cell types

RNA: detected in 37 tissues
Protein: detected in 20 cell types

RNA: detected in 37 tissues
Protein: detected in 47 cell types

RNA: detected in 37 tissues
Protein: detected in 21 cell types

Retrieval

HIER pH6

Antibody dilution

1:100

1:75

1:85

1:200

1:50

Literature conformity

Consistent with extensive gene/protein characterization data.

Partly consistent with extensive gene/protein characterization data.

Consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Mainly not consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA027873

Antibody HPA042128

Antibody HPA049883

Antibody HPA051156

Antibody CAB003860

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

90.6, 87.4, 86.9

90.6, 89.9, 87.4, 86.9

90.6, 89.9, 87.4, 86.9, 16.7

90.6, 87.4, 86.9, 26.2, 16.7, 14.7

Antibody dilution

1:250

1:260

1:190

1:500

PROTEIN ARRAY

Antibody HPA027873

Antibody HPA042128

Antibody HPA049883

Antibody HPA051156

Antibody CAB003860

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:500

1:3000

1:2100

1:1550

ANTIGEN INFORMATION

Antibody HPA027873

Antibody HPA042128

Antibody HPA049883

Antibody HPA051156

Antibody CAB003860

Antigen

Recombinant protein fragment

Synthetic peptide

Length (aa)

Antigen sequence

YPQNPDHVLDQDGEFDLDETMDVARHVEELLRRPMDSLDSRLSPPAGLFT
SARGSLS

EGSLDVLQSWCEKLAEIIWQNRQQIRRAEHLCQQLPIPGPVEEMLAEVNA
TITDIISALVTSTFIIEKQPPQVL

KTQTKFAATVRLLVGGKLNVHMNPPQVKATIISEQQAKSLLKNENTRN

LEAWLQREAQTLQQYRVELAEKHQKTLQLLRKQQTIILDDELIQWKRRQ

Matching transcripts

STAT5A-001 - ENSP00000341208 [100%]
STAT5A-007 - ENSP00000468749 [100%]
STAT5A-008 - ENSP00000443107 [100%]
STAT5A-009 - ENSP00000465437 [100%]

STAT5A-001 - ENSP00000341208 [100%]
STAT5A-007 - ENSP00000468749 [100%]
STAT5A-008 - ENSP00000443107 [100%]
STAT5A-009 - ENSP00000465437 [100%]
STAT5A-012 - ENSP00000464730 [100%]

Other gene match

STAT5B - ENSG00000173757 [100%]

ANTIGEN VIEW

STAT5A-001	STAT5A-007	STAT5A-008	STAT5A-009	STAT5A-012

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.