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SGPP1

ANTIBODY INFORMATION
 
 

Antibody HPA053149

  

Antibody HPA064908

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA053149   HPA064908  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 10   14  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA053149

  

Antibody HPA064908

  
Immunocytochemistry


Validated
  

Validated
  
Immunohistochemistry

Approved    
Western blot

Supported   Supported  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA053149

  

Antibody HPA064908

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA064908.		  
Validated

Antibody staining overlaps with antibody HPA053149.		  
 

Standard validation

 Uncertain   Uncertain  

   

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleus.  

Antibody dilution

 1:49   1:9  

Literature conformity

 The subcellular location is not consistent with literature.   The subcellular location is not consistent with literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA053149

  

Antibody HPA064908

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 50 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:300    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA053149

  

Antibody HPA064908

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

   

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 49.1   49.1  

Antibody dilution

 1:250   1:80  

PROTEIN ARRAY
 
 

Antibody HPA053149

  

Antibody HPA064908

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:1950   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA053149

  

Antibody HPA064908

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 32   20  

Antigen sequence

 
KITIPLACKIFNIPCDDIRKARQHMEVELPYR
  
YPFVDLIDNFNQTHKYAPFI
 

Matching transcripts

 SGPP1-001 - ENSP00000247225 [100%]
  SGPP1-001 - ENSP00000247225 [100%]
 

Other gene match

    
ANTIGEN VIEW
SGPP1-001
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.