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Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cell in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic and membranous positivity in cell in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic and extracellular positivity in cells in tubules.
Expression
RNA: detected in 0 tissues Protein: detected in 24 cell types
RNA: detected in 0 tissues Protein: detected in 17 cell types
RNA: detected in 0 tissues Protein: detected in 48 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:15
1:120
1:100
Literature conformity
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
Partly consistent with gene/protein characterization data.
RNA consistency
Not consistent with RNA expression data.
Not consistent with RNA expression data.
Not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA043362
Antibody HPA044237
Antibody CAB025858
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
58.8
58.8
58.8
Antibody dilution
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA043362
Antibody HPA044237
Antibody CAB025858
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
