Fields »
ABOUT
HELP
BLOG
ECHS1

ANTIBODY INFORMATION
 
 

Antibody HPA021995

  

Antibody HPA022476

  

Antibody CAB003783

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   HUPO-liver
  

Product name

 HPA021995   HPA022476    

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 5   5   2  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA021995

  

Antibody HPA022476

  

Antibody CAB003783

  
Immunocytochemistry


Validated
  

Validated
   Supported  
Immunohistochemistry


Validated
  

Validated
   Supported  
Western blot

Supported   Supported   Supported  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA021995

  

Antibody HPA022476

  

Antibody CAB003783

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA022476.		  
Validated

Antibody staining overlaps with antibody HPA021995.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.   Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.   Immunofluorescent staining of human cell line A-431 shows localization to mitochondria.  

Antibody dilution

 1:59   1:101   1:2000  

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA021995

  

Antibody HPA022476

  

Antibody CAB003783

  

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		  
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 

  
 

    

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA022476 across 44 cell lines.		   Distribution of protein expression (antibody staining).
Pearson correlation with HPA021995 across 44 cell lines.		    
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.   Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 78 cell types		   RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 73 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:1000   1:900   1:24000  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA021995

  

Antibody HPA022476

  

Antibody CAB003783

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 110, 82, 49.3, 32.2, 25.5, 17.6
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 31.4   31.4   31.4  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA021995

  

Antibody HPA022476

  

Antibody CAB003783

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA021995

  

Antibody HPA022476

  

Antibody CAB003783

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Native protein  

Length (aa)

 118   142    

Antigen sequence

 
ANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDP
AVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPV
IAAVNGYAFGGGCELAMM
  
CDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRI
SAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAF
EMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ
  

											 
																				

																		

										
											
Matching transcripts

												
																					ECHS1-001 - ENSP00000357535 [99%] 

											 
																						ECHS1-001 - ENSP00000357535 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
ECHS1-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

					Contact
				

				
				

					
					
					
					
				

				
			


			

				

					The Project
				
			
				
			

			
			

			
				

					The Human Protein Atlas
				

				
			

			
			

			

						The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.