SPECC1 - Antibodies - The Human Protein Atlas

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SPECC1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA021421

Antibody HPA021430

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA021421

HPA021430

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA021421

Antibody HPA021430

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOHISTOCHEMISTRY

Antibody HPA021421

Antibody HPA021430

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA021430 across 73 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA021421 across 73 cell types.

Standard validation

Approved

Figure description

Immunohistochemical staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.

Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferus ducts.

Expression

RNA: detected in 37 tissues
Protein: detected in 70 cell types

RNA: detected in 37 tissues
Protein: detected in 66 cell types

Retrieval

HIER pH6

Antibody dilution

1:325

1:100

Literature conformity

Partly consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA021421

Antibody HPA021430

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

118.6, 110.2, 87.9, 79.5, 79

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA021421

Antibody HPA021430

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA021421

Antibody HPA021430

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

DTEPMIRALEEKNKNFQKELSDLEEENRVLKEKLIYLEHSPNSEGAASHT
GDSSCPTSITQESSFGSPTGNQMSSD

APREFSVTVSRERSVPRGPSNPRKSVSSPTSSNTPTPTKHLRTPSTKPKQ
ENEGGEKAALESQVRELLAEAKAKDSEINRLRSELKKYKEKRTLNA

Matching transcripts

SPECC1-002 - ENSP00000378900 [100%]
SPECC1-003 - ENSP00000378893 [100%]
SPECC1-004 - ENSP00000378901 [100%]
SPECC1-018 - ENSP00000261503 [100%]
SPECC1-026 - ENSP00000378896 [100%]
SPECC1-027 - ENSP00000378898 [100%]

Other gene match

ANTIGEN VIEW

SPECC1-002	SPECC1-003	SPECC1-004	SPECC1-018	SPECC1-026	SPECC1-027

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.