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Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.
Antibody dilution
1:50
1:23
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA000382
Antibody HPA000383
Antibody HPA001679
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human oral mucosa shows moderate nuclear positivity in squamous epithelial cells.
Immunohistochemical staining of human thyroid gland shows strong nuclear positivity in glandular cells.
Expression
RNA: detected in 28 tissues Protein: detected in 47 cell types
RNA: detected in 28 tissues Protein: detected in 74 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:250
1:25
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA000382
Antibody HPA000383
Antibody HPA001679
Standard validation
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
