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Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm & nuclear bodies.
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & nuclear bodies.
Antibody dilution
1:96
1:63
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
WESTERN BLOT
Antibody HPA041417
Antibody HPA041484
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
176.8
176.8
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA041417
Antibody HPA041484
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
