MTUS1 - Antibodies - The Human Protein Atlas

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MTUS1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA067903

Antibody HPA069795

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA067903

HPA069795

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA067903

Antibody HPA069795

Immunocytochemistry

Western blot

Uncertain

Protein array

Supported

IMMUNOCYTOCHEMISTRY

Antibody HPA067903

Antibody HPA069795

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA069795.

Validated

Antibody staining overlaps with antibody HPA067903.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A549 shows localization to microtubules.

Immunofluorescent staining of human cell line A549 shows localization to nucleoli & microtubules.

Antibody dilution

1:60

1:196

Literature conformity

The subcellular location is supported by literature.

WESTERN BLOT

Antibody HPA067903

Antibody HPA069795

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.

Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.

Figure description

Target mass (kDa)

141.4, 135.7

Antibody dilution

1:200

1:430

PROTEIN ARRAY

Antibody HPA067903

Antibody HPA069795

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2400

1:7800

ANTIGEN INFORMATION

Antibody HPA067903

Antibody HPA069795

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

STPVLEPTKVTFSVSPIEATEKCKKVEKGNRGLKNIPDSKEAPVNLCKPS
LGKSTIKTNTPIGCKVRKTEIISYPRPNFKNVKAK

SGKVTSEYTDGSQQRLVGEKETQALTPVSDGMEVPNDSALQEFFCLSHDE
SNSEPHSQSSYRHKEMGQNLRETVSYCLIDDECPLMVPAFDKSEAQVLN

Matching transcripts

MTUS1-001 - ENSP00000262102 [100%]
MTUS1-004 - ENSP00000430167 [100%]
MTUS1-201 - ENSP00000371293 [100%]

Other gene match

ANTIGEN VIEW

MTUS1-001	MTUS1-004	MTUS1-201

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.