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ANTIBODY INFORMATION
 
 

Antibody HPA064599

  

Antibody CAB026066

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA064599   AF2945  

Host species

 Rabbit   Goat  

Clonality

 pAb   msAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity  

Other gene match

    

Released in version

 16   16  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA064599

  

Antibody CAB026066

  
Immunocytochemistry

Supported    
Immunohistochemistry

  Supported  
Western blot

Uncertain   Uncertain  
Protein array

Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA064599

  

Antibody CAB026066

  

Standard validation

 Supported    

   

Figure description

 Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & cytosol.    

Antibody dilution

 1:200    

Literature conformity

 The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA064599

  

Antibody CAB026066

  

Standard validation

   Supported  

   

Figure description

   Immunohistochemical staining of human retina shows strong nuclear positivity in rods.  

Expression

   RNA: detected in 23 tissues
Protein: detected in 0 cell types		  

Retrieval

   HIER pH6  

Antibody dilution

   1:150  

Literature conformity

   Consistent with extensive gene/protein characterization data.  

RNA consistency

   No internal RNA expression data available for correlation.  

WESTERN BLOT
 
 

Antibody HPA064599

  

Antibody CAB026066

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 25.9   25.9, 11.3, 6.8  

Antibody dilution

 1:770   1:500  

PROTEIN ARRAY
 
 

Antibody HPA064599

  

Antibody CAB026066

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:9200    

ANTIGEN INFORMATION
 
 

Antibody HPA064599

  

Antibody CAB026066

  

Antigen

 Recombinant protein fragment   Recombinant protein  

Length (aa)

 71    

Antigen sequence

 
FSEPGMVGATEGTRPGLEELYWLATLQQQLGAGEALGLSPEEAMELLQGQ
GPVPVDGPHGYYPGSPEETGA
  

											 
																				

																		

										
											
Matching transcripts

												
																					NRL-002 - ENSP00000380197 [100%] 
NRL-003 - ENSP00000380193 [100%] 
NRL-004 - ENSP00000454062 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
NRL-002
NRL-003
NRL-004
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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