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Immunofluorescent staining of human cell line A-431 shows localization to nucleus, plasma membrane & cytosol.
Antibody dilution
1:106
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA041153
Antibody HPA042031
Formal validation: Independent
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA042031 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA041153 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA042031 across 72 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA041153 across 72 cell types.
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelium.
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 73 cell types
RNA: detected in 37 tissues Protein: detected in 68 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:1500
1:225
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA041153
Antibody HPA042031
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415258)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415258)
Target mass (kDa)
25.1, 24.8, 18.7
25.1, 18.7
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA041153
Antibody HPA042031
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
