CLIP1 - Antibodies - The Human Protein Atlas

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CLIP1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA026678

Antibody CAB004399

Provider

Atlas Antibodies
Sigma-Aldrich

Santa Cruz Biotechnology

Product name

HPA026678

sc-28325

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA026678

Antibody CAB004399

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA026678

Antibody CAB004399

Standard validation

Approved

Figure description

Immunofluorescent staining of human cell line SiHa shows localization to microtubule ends.

Antibody dilution

1:54

Literature conformity

The subcellular location is partly supported by literature or no literature is available.

IMMUNOHISTOCHEMISTRY

Antibody HPA026678

Antibody CAB004399

Standard validation

Approved

Figure description

Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Immunohistochemical staining of human prostate shows strong cytoplasmic and nuclear positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 69 cell types

RNA: detected in 37 tissues
Protein: detected in 55 cell types

Retrieval

HIER pH6

Antibody dilution

1:450

1:75

Literature conformity

Partly consistent with extensive gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Mainly not consistent with RNA expression data.

WESTERN BLOT

Antibody HPA026678

Antibody CAB004399

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: U-251 MG
Lane 3: Human Plasma
Lane 4: Liver
Lane 5: Tonsil
Lane 6: RT4

Target mass (kDa)

162.2, 161, 156.8, 148.1, 116.3

162.2, 161, 156.8, 148.1, 116.3, 89.6, 46.2, 13.5, 11.5, 8.9

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA026678

Antibody CAB004399

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA026678

Antibody CAB004399

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

150

Antigen sequence

KNLELQLKENKRQLSSSSGNTDTQADEDERAQESQIDFLNSVIVDLQRKN
QDLKMKVEMMSEAALNGNGDDLNNYDSDDQEKQSKKKPRLFCDICDCFDL
HDTEDCPTQAQMSEDPPHSTHHGSRGEERPYCEICEMFGHWATNCNDDET

Matching transcripts

CLIP1-001 - ENSP00000303585 [100%]
CLIP1-002 - ENSP00000445531 [100%]
CLIP1-003 - ENSP00000439093 [100%]
CLIP1-007 - ENSP00000355314 [100%]
CLIP1-008 - ENSP00000438743 [100%]
CLIP1-201 - ENSP00000351665 [100%]
CLIP1-202 - ENSP00000479322 [100%]

Other gene match

ANTIGEN VIEW