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Immunofluorescent staining of human cell line A-431 shows localization to nucleus & mitochondria.
Antibody dilution
1:79
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA038194
Antibody HPA057595
Standard validation
Uncertain
Figure description
Immunohistochemical staining of human urinary bladder shows distinct positivity in endothelial cells.
Expression
RNA: detected in 37 tissues Protein: detected in 20 cell types
Retrieval
HIER pH6
Antibody dilution
1:300
Literature conformity
No avaliable gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA038194
Antibody HPA057595
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Uncertain
Analysis performed using a standard panel of samples. Only bands not corresponding to the predicted size.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY407691)
Target mass (kDa)
18.8, 15.4, 10.4
18.8
Antibody dilution
1:250
1:260
PROTEIN ARRAY
Antibody HPA038194
Antibody HPA057595
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
