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UBA1

ANTIBODY INFORMATION
 
 

Antibody HPA000289

  

Antibody HPA001506

  

Antibody CAB019435

  

Antibody CAB073410

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Cell Signaling Technology, Inc
   NCI-CPTC
  

Product name

 HPA000289   HPA001506   4890   CPTC-UBA1-1  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known   Protein A/G  

Other gene match

        

Released in version

 1   1   5   13  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA000289

  

Antibody HPA001506

  

Antibody CAB019435

  

Antibody CAB073410

  
Immunocytochemistry

  Supported   Supported    
Immunohistochemistry

Approved     Approved   Approved  
Western blot

Supported   Supported   Supported   Supported  
Protein array

Supported   Supported      

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA000289

  

Antibody HPA001506

  

Antibody CAB019435

  

Antibody CAB073410

  

Standard validation

   Supported   Supported    

       

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.   Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.    

Antibody dilution

   1:15   1:25    

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA000289

  

Antibody HPA001506

  

Antibody CAB019435

  

Antibody CAB073410

  

Standard validation

 Approved     Approved   Approved  

       

Figure description

 Immunohistochemical staining of human cerebral cortex shows moderate nuclear positivity in glial cells.     Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.   Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 32 cell types		     RNA: detected in 37 tissues
Protein: detected in 76 cell types		   RNA: detected in 37 tissues
Protein: detected in 75 cell types		  

Retrieval

 HIER pH6     HIER pH6   HIER pH6  

Antibody dilution

 1:75     1:100   1:4000  

Literature conformity

 Partly consistent with extensive gene/protein characterization data.     Partly consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly not consistent with RNA expression data.     Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA000289

  

Antibody HPA001506

  

Antibody CAB019435

  

Antibody CAB073410

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

       

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 117.8, 56.9   117.8, 56.9   117.8, 56.9, 30.8, 29.2, 25.1, 20.7, 18.6   117.8, 56.9, 30.8, 29.2, 25.1, 20.7, 18.6  

Antibody dilution

 1:250   1:250   1:500   1:500  

PROTEIN ARRAY
 
 

Antibody HPA000289

  

Antibody HPA001506

  

Antibody CAB019435

  

Antibody CAB073410

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		      

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.      

Antibody dilution

 1:2000   1:500      

ANTIGEN INFORMATION
 
 

Antibody HPA000289

  

Antibody HPA001506

  

Antibody CAB019435

  

Antibody CAB073410

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Synthetic peptide   Recombinant protein  

Length (aa)

 112   150      

Antigen sequence

 
LAAPRHQYYNQEWTLWDRFEVQGLQPNGEEMTLKQFLDYFKTEHKLEITM
LSQGVSMLYSFFMPAAKLKERLDQPMTEIVSRVSKRKLGRHVRALVLELC
CNDESGEDVEVP
  
LYKVVQGHRQLDSYKNGFLNLALPFFGFSEPLAAPRHQYYNQEWTLWDRF
EVQGLQPNGEEMTLKQFLDYFKTEHKLEITMLSQGVSMLYSFFMPAAKLK
ERLDQPMTEIVSRVSKRKLGRHVRALVLELCCNDESGEDVEVPYVRYTIR
  

											 
																						

											 
																				

																		

										
											
Matching transcripts

												
																					UBA1-001 - ENSP00000338413 [100%] 
UBA1-002 - ENSP00000366568 [100%] 
UBA1-003 - ENSP00000366481 [100%] 

											 
																						UBA1-001 - ENSP00000338413 [100%] 
UBA1-002 - ENSP00000366568 [100%] 
UBA1-003 - ENSP00000366481 [100%] 

											 
																						
											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
UBA1-001
UBA1-002
UBA1-003
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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