GFAP - Antibodies - The Human Protein Atlas

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GFAP

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA056030

Antibody HPA063513

Antibody CAB000039

Provider

Atlas Antibodies
Sigma-Aldrich

DakoCytomation

Product name

HPA056030

HPA063513

Z0334

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA056030

Antibody HPA063513

Antibody CAB000039

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA056030

Antibody HPA063513

Antibody CAB000039

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line HEK 293 shows localization to intermediate filaments.

Antibody dilution

1:55

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA056030

Antibody HPA063513

Antibody CAB000039

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA063513 across 75 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA056030 across 75 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human cerebral cortex shows distinct cytoplasmic positivity in astrocytes and neuropil.

Immunohistochemical staining of human cerebral cortex shows moderate positivity in astrocytes.

Immunohistochemical staining of human cerebral cortex shows distinct positivity in astrocytes.

Expression

RNA: detected in 32 tissues
Protein: detected in 4 cell types

RNA: detected in 32 tissues
Protein: detected in 8 cell types

RNA: detected in 32 tissues
Protein: detected in 6 cell types

Retrieval

HIER pH6

Proteinase K.

Antibody dilution

1:550

1:18000

1:2000

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Standard validation

Supported

Figure description

Subsets of astrocytes were distinctly stained, most abundant in hippocampus, piriform area and rostral migratory stream.

WESTERN BLOT

Antibody HPA056030

Antibody HPA063513

Antibody CAB000039

Standard validation

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

49.9

50.3, 49.9, 49.5, 29.8, 28.5, 21.3

50.3, 49.9, 49.5, 29.8, 28.5, 22.4, 21.3, 11.4, 7.1, 6.6, 1.4, 1.1

Antibody dilution

1:180

1:230

1:500

PROTEIN ARRAY

Antibody HPA056030

Antibody HPA063513

Antibody CAB000039

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:2200

1:2800

ANTIGEN INFORMATION

Antibody HPA056030

Antibody HPA063513

Antibody CAB000039

Antigen

Recombinant protein fragment

Native protein

Length (aa)

Antigen sequence

LEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGE
VIKESKQEHKD

NKALAAELNQLRAKEPTKLADVYQAELRELRLRLDQLTANSARLEVERD

Matching transcripts

GFAP-001 - ENSP00000253408 [100%]

GFAP-001 - ENSP00000253408 [100%]
GFAP-002 - ENSP00000403962 [100%]
GFAP-005 - ENSP00000465320 [100%]
GFAP-006 - ENSP00000466163 [100%]
GFAP-018 - ENSP00000465629 [100%]
GFAP-024 - ENSP00000468500 [100%]

Other gene match

ANTIGEN VIEW

GFAP-001	GFAP-002	GFAP-005	GFAP-006	GFAP-018	GFAP-024

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.