HACL1 - Antibodies - The Human Protein Atlas

We use cookies to enhance the usability of our website. If you continue, we'll assume that you are happy to receive all cookies. More information. Don't show this again.

HACL1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA035496

Antibody HPA035497

Antibody HPA073760

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA035496

HPA035497

HPA073760

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA035496

Antibody HPA035497

Antibody HPA073760

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA035496

Antibody HPA035497

Antibody HPA073760

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line PC-3 shows localization to vesicles.

Antibody dilution

1:53

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA035496

Antibody HPA035497

Antibody HPA073760

Standard validation

Approved

Figure description

Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 55 cell types

RNA: detected in 37 tissues
Protein: detected in 54 cell types

Retrieval

HIER pH6

Antibody dilution

1:150

1:25

Literature conformity

No avaliable gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA035496

Antibody HPA035497

Antibody HPA073760

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Uncertain

Analysis performed using a standard panel of samples.
No bands detected.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

63.7, 61.1, 56.7, 54.7, 37.6

63.7, 61.1, 56.7, 26.4

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA035496

Antibody HPA035497

Antibody HPA073760

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

1:2150

ANTIGEN INFORMATION

Antibody HPA035496

Antibody HPA035497

Antibody HPA073760

Antigen

Recombinant protein fragment

Length (aa)

Antigen sequence

CVEGDSAFGFSGMEVETICRYNLPIILLVVNNNGIYQGFDTDTWKEMLKF
QDATAVVPPMCLLPNSHYEQVMTAFGGKGYFVQTPEELQ

YGRPGACYVDIPADFVNLQVNVNSIKYMERCMSPPISMAETSAVCTAASV
IRNAKQPLLIIGKGAAYAHAEESIKKLVEQYKLPFLPTPMGKGVV

QLGIKYIGMRNEQAACYAASAIGYLTSRPGVCLVVSGPGLIHALGGMANA
NMNCWPLLVIGGSSERNQETMGAFQEFPQVEA

Matching transcripts

HACL1-001 - ENSP00000323811 [100%]
HACL1-002 - ENSP00000403656 [100%]
HACL1-003 - ENSP00000395278 [100%]
HACL1-005 - ENSP00000390699 [100%]
HACL1-007 - ENSP00000404883 [100%]

HACL1-001 - ENSP00000323811 [100%]
HACL1-005 - ENSP00000390699 [100%]
HACL1-007 - ENSP00000404883 [100%]
HACL1-008 - ENSP00000391393 [100%]

HACL1-001 - ENSP00000323811 [100%]
HACL1-007 - ENSP00000404883 [96%]

Other gene match

ANTIGEN VIEW

HACL1-001	HACL1-002	HACL1-003	HACL1-005	HACL1-007	HACL1-008

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.