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NAPSA

ANTIBODY INFORMATION
 
 

Antibody HPA045280

  

Antibody HPA047236

  

Antibody HPA047744

  

Antibody CAB009591

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA045280   HPA047236   HPA047744   NCL-Napsin A  

Host species

 Rabbit   Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

        

Released in version

 12   12   12   4  

References

        

VALIDATION SUMMARY
 
 

Antibody HPA045280

  

Antibody HPA047236

  

Antibody HPA047744

  

Antibody CAB009591

  
Immunohistochemistry


Validated
   Supported  

Validated
  

Validated
  
Western blot

Uncertain   Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported   Supported    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA045280

  

Antibody HPA047236

  

Antibody HPA047744

  

Antibody CAB009591

  

Formal validation: Orthogonal

      
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

     
 

 

  

Figure description

       Distribution of protein expression (antibody staining) and RNA expression (TPM) across 39 cell lines.  
 

Formal validation: Independent
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    
Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.		    

 

    
 

    

Figure description

 Distribution of protein expression (antibody staining).
Pearson correlation with HPA047744 across 46 cell lines.		     Distribution of protein expression (antibody staining).
Pearson correlation with HPA045280 across 46 cell lines.		    
 

Standard validation

 Supported   Supported   Supported   Supported  

       

Figure description

 Immunohistochemical staining of human lung shows strong cytoplasmic positivity in pneumocytes and macrophages.   Immunohistochemical staining of human lung shows strong cytoplasmic positivity in type 2 pneumocytes.   Immunohistochemical staining of human lung shows strong cytoplasmic positivity in granular pattern in type II pneumocytes.   Immunohistochemical staining of human lung shows strong cytoplasmic positivity in pneumocytes and macrophages.  

Expression

 RNA: detected in 12 tissues
Protein: detected in 16 cell types		   RNA: detected in 12 tissues
Protein: detected in 23 cell types		   RNA: detected in 12 tissues
Protein: detected in 3 cell types		   RNA: detected in 12 tissues
Protein: detected in 4 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:25   1:75   1:150  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA045280

  

Antibody HPA047236

  

Antibody HPA047744

  

Antibody CAB009591

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

       

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 45.4, 29.1   45.4, 29.1   45.4, 29.1   45.4, 29.1  

Antibody dilution

 1:250   1:110   1:80   1:500  

PROTEIN ARRAY
 
 

Antibody HPA045280

  

Antibody HPA047236

  

Antibody HPA047744

  

Antibody CAB009591

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

       

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:900   1:650    

ANTIGEN INFORMATION
 
 

Antibody HPA045280

  

Antibody HPA047236

  

Antibody HPA047744

  

Antibody CAB009591

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 31   36   45    

Antigen sequence

 
SDPAHYIPPLTFVPVTVPAYWQIHMERVKVG
  
DGILGLGFPILSVEGVRPPLDVLVEQGLLDKPVFSF
  
GTKFAIQYGTGRVDGILSEDKLTIGGIKGASVIFGEALWEPSLVF
   

Matching transcripts

 NAPSA-001 - ENSP00000253719 [100%]
NAPSA-006 - ENSP00000469061 [100%]
  NAPSA-001 - ENSP00000253719 [97%]
NAPSA-006 - ENSP00000469061 [97%]
  NAPSA-001 - ENSP00000253719 [100%]
NAPSA-006 - ENSP00000469061 [100%]
   

Other gene match

        
ANTIGEN VIEW
NAPSA-001
NAPSA-006
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.