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TOP2A

ANTIBODY INFORMATION
 
 

Antibody HPA006458

  

Antibody HPA026773

  

Antibody CAB002448

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Novocastra
  

Product name

 HPA006458   HPA026773   NCL-TOPOIIA  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Not known  

Other gene match

      

Released in version

 5   5   1  

References

 2      

VALIDATION SUMMARY
 
 

Antibody HPA006458

  

Antibody HPA026773

  

Antibody CAB002448

  
Immunocytochemistry

Supported     Supported  
Immunohistochemistry


Validated
  

Validated
  

Validated
  
Western blot

Supported   Uncertain   Supported  
Protein array

Supported   Approved    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA006458

  

Antibody HPA026773

  

Antibody CAB002448

  

Standard validation

 Supported     Supported  

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.     Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.  

Antibody dilution

 1:38     1:8  

Literature conformity

 The subcellular location is supported by literature.     The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA006458

  

Antibody HPA026773

  

Antibody CAB002448

  

Formal validation: Orthogonal
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  
Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.		  

 

 

  
 

 

  
 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.   Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human lymph node shows strong nuclear positivity in reaction center cells   Immunohistochemical staining of human testis shows strong nuclear positivity in cells in seminiferus ducts.   Immunohistochemical staining of human duodenum shows strong nuclear positivity in subset of glandular cells.  

Expression

 RNA: detected in 28 tissues
Protein: detected in 45 cell types		   RNA: detected in 28 tissues
Protein: detected in 44 cell types		   RNA: detected in 28 tissues
Protein: detected in 41 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:200   1:1000   1:30  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.   Consistent with RNA expression data.   Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA006458

  

Antibody HPA026773

  

Antibody CAB002448

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		  

     

Figure description

 Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 174.4   174.4   174.4, 17.1, 3.1  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA006458

  

Antibody HPA026773

  

Antibody CAB002448

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA006458

  

Antibody HPA026773

  

Antibody CAB002448

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Not known  

Length (aa)

 142   97    

Antigen sequence

 
DASPPKTKTSPKLSNKELKPQKSVVSDLEADDVKGSVPLSSSPPATHFPD
ETEITNPVPKKNVTVKKTAAKSQSSTSTTGAKKRAAPKGTKRDPALNSGV
SQKPDPAKTKNRRKRKPSTSDDSDSNFEKIVSKAVTSKKSKG
  
RGQRVIPRITIEMKAEAEKKNKKKIKNENTEGSPQEDGVELEGLKQRLEK
KQKREPGTKTKKQTTLAFKPIKKGKKRNPWSDSESDRSSDESNFDVP
  

											 
																				

																		

										
											
Matching transcripts

												
																					TOP2A-001 - ENSP00000411532 [100%] 

											 
																						TOP2A-001 - ENSP00000411532 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
TOP2A-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
											

									
										
										
										
										
RELEVANT PUBLICATIONS

											
								

								

									 
								

								

									 
																			
											
											
											
Antibody HPA006458

												
										 
																				
											
											
											
Antibody HPA026773

												
										 
																				
											
											
											
Antibody CAB002448

												
										 
																		

																	

										
											
1.

												
																						Tumour expression of bladder cancer-associated urinary proteins

	Lindén M et al

	BJU Int 2013;112(3):407-15

			
											 
																						
											 
																						
											 
																				

																		

										
											
2.

												
																						Antibodies biotinylated using a synthetic Z-domain from protein A provide stringent in situ protein detection

	Andersson S et al

	J Histochem Cytochem 2013;61(11):773-84

			
											 
																						
											 
																						
											 
																				

															
				
						

				 
			

		

			
	











	

		


			

				

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