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PDHA1

ANTIBODY INFORMATION
 
 

Antibody HPA047487

  

Antibody HPA047864

  

Antibody HPA063053

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA047487   HPA047864   HPA063053  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

 PDHA2 - ENSG00000163114 [100%]
   PDHA2 - ENSG00000163114 [100%]
   PDHA2 - ENSG00000163114 [100%]
  

Released in version

 12   12   13  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA047487

  

Antibody HPA047864

  

Antibody HPA063053

  
Immunocytochemistry

  Supported    
Immunohistochemistry

Approved   Approved   Approved  
Western blot

Uncertain   Supported   Uncertain  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA047487

  

Antibody HPA047864

  

Antibody HPA063053

  

Standard validation

   Supported    

     

Figure description

   Immunofluorescent staining of human cell line HeLa shows localization to mitochondria.    

Antibody dilution

   1:34    

Literature conformity

   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA047487

  

Antibody HPA047864

  

Antibody HPA063053

  

Standard validation

 Approved   Approved   Approved  

     

Figure description

 Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in subset of cells in seminiferus ducts.   Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in Leydig cells.   Immunohistochemical staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts, Leydig cells were strongly stained.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 20 cell types		   RNA: detected in 37 tissues
Protein: detected in 44 cell types		   RNA: detected in 37 tissues
Protein: detected in 77 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:50   1:75   1:900  

Literature conformity

 Not consistent with gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Not consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA047487

  

Antibody HPA047864

  

Antibody HPA063053

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		    

Target mass (kDa)

 47.6, 44.1, 43.3, 42.9, 40.2, 22.8, 22.3, 19.8   47.6, 44.1, 43.3, 42.9, 40.2, 22.8, 22.3, 19.8, 13.8   47.6, 44.1, 43.3, 42.9, 40.2  

Antibody dilution

 1:220   1:170   1:150  

PROTEIN ARRAY
 
 

Antibody HPA047487

  

Antibody HPA047864

  

Antibody HPA063053

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:500   1:1350   1:1750  

ANTIGEN INFORMATION
 
 

Antibody HPA047487

  

Antibody HPA047864

  

Antibody HPA063053

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 35   27   29  

Antigen sequence

 
ILAELTGRRGGCAKGKGGSMHMYTKNFYGGNGIVG
  
NDATFEIKKCDLYLLEEGPPVTTVLTR
  
YCRSGKGPILMELQTYRYHGHSMSDPGVS
 

Matching transcripts

 PDHA1-001 - ENSP00000369133 [94%]
PDHA1-004 - ENSP00000394382 [94%]
PDHA1-008 - ENSP00000348062 [94%]
PDHA1-201 - ENSP00000369134 [94%]
PDHA1-202 - ENSP00000440761 [94%]
PDHA1-203 - ENSP00000438550 [94%]
PDHA1-009 - ENSP00000406473 [91%]
  PDHA1-001 - ENSP00000369133 [93%]
PDHA1-004 - ENSP00000394382 [93%]
PDHA1-008 - ENSP00000348062 [93%]
PDHA1-009 - ENSP00000406473 [93%]
PDHA1-010 - ENSP00000404616 [93%]
PDHA1-201 - ENSP00000369134 [93%]
PDHA1-202 - ENSP00000440761 [93%]
PDHA1-203 - ENSP00000438550 [93%]
  PDHA1-004 - ENSP00000394382 [100%]
PDHA1-201 - ENSP00000369134 [100%]
PDHA1-202 - ENSP00000440761 [100%]
PDHA1-203 - ENSP00000438550 [100%]
 

Other gene match

 PDHA2 - ENSG00000163114 [100%]
   PDHA2 - ENSG00000163114 [100%]
   PDHA2 - ENSG00000163114 [100%]
  
ANTIGEN VIEW
PDHA1-001
PDHA1-004
PDHA1-008
PDHA1-009
PDHA1-010
PDHA1-201
PDHA1-202
PDHA1-203
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.