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Immunofluorescent staining of human cell line U-251 MG shows localization to intermediate filaments.
Antibody dilution
1:85
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA015870
Antibody HPA021872
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in glomeruli.
Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.
Expression
RNA: detected in 35 tissues Protein: detected in 74 cell types
RNA: detected in 35 tissues Protein: detected in 39 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:300
1:150
Literature conformity
Partly consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly not consistent with RNA expression data.
Mainly not consistent with RNA expression data.
WESTERN BLOT
Antibody HPA015870
Antibody HPA021872
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401950)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401950)
Target mass (kDa)
80.6, 74.6
80.6, 74.6
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA015870
Antibody HPA021872
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
