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TERF2

ANTIBODY INFORMATION
 
 

Antibody HPA001907

  

Antibody HPA002735

  

Antibody CAB010451

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Santa Cruz Biotechnology
  

Product name

 HPA001907   HPA002735   sc-9143  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 2   4   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA001907

  

Antibody HPA002735

  

Antibody CAB010451

  
Immunocytochemistry


Validated
      
Immunohistochemistry

Supported   Supported   Supported  
Western blot

Supported   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA001907

  

Antibody HPA002735

  

Antibody CAB010451

  

Formal validation: Genetic
Validated

Significant downregulation > 25% by both siRNA:s.		      

 

Relative fluorescence intensity (RFI)

      

Figure description

 Nuclear region of segmented cells in 10x-images      

Antibody dilution

 1:37      
 

Standard validation

 Supported      

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.      

Antibody dilution

 1:38      

Literature conformity

 The subcellular location is supported by literature.      

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA001907

  

Antibody HPA002735

  

Antibody CAB010451

  

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center cells and non-germinal center cells.   Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center cells and non-germinal center cells.   Immunohistochemical staining of human tonsil shows nuclear positivity in germinal center cells and non-germinal center cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 64 cell types		   RNA: detected in 37 tissues
Protein: detected in 68 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:250   1:100   1:25  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Partly consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA001907

  

Antibody HPA002735

  

Antibody CAB010451

  

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		  

     

Figure description

 Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 17.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: A-431
Lane 5: Liver
Lane 6: Tonsil		      

Target mass (kDa)

 59.6, 31.5, 26.7, 15.4   59.6, 32.3, 31.5   59.6, 32.3, 31.5, 26.7, 22.9, 15.4, 9.7, 5.7  

Antibody dilution

 1:250   1:250   1:500  

PROTEIN ARRAY
 
 

Antibody HPA001907

  

Antibody HPA002735

  

Antibody CAB010451

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:3000    

ANTIGEN INFORMATION
 
 

Antibody HPA001907

  

Antibody HPA002735

  

Antibody CAB010451

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 123   132    

Antigen sequence

 
STGKEDKQPAPGPVEKPPREPARQLRNPPTTIGMMTLKAAFKTLSGAQDS
EAAFAKLDQKDLVLPTQALPASPALKNKRPRKDENESSAPADGEGGSELQ
PKNKRMTISRLVLEEDSQSTEPS
  
DIMQALLVRPLGKEHTVSRLLRVMQCLSRIEEGENLDCSFDMEAELTPLE
SAINVLEMIKTEFTLTEAVVESSRKLVKEAAVIICIKNKEFEKASKILKK
HMSKDPTTQKLRNDLLNIIREKNLAHPVIQNF
   

Matching transcripts

 TERF2-001 - ENSP00000254942 [100%]
TERF2-011 - ENSP00000475507 [100%]
TERF2-006 - ENSP00000456022 [90%]
TERF2-012 - ENSP00000462726 [87%]
  TERF2-001 - ENSP00000254942 [100%]
TERF2-002 - ENSP00000454955 [100%]
TERF2-006 - ENSP00000456022 [97%]
   

Other gene match

      
ANTIGEN VIEW
TERF2-001
TERF2-002
TERF2-006
TERF2-011
TERF2-012
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.