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Antibody staining overlaps with GFP tagged protein
Figure description
Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus & cytoplasm and GFP expression in nucleus but excluded from the nucleoli.
Antibody dilution
1:8
Standard validation
Supported
Supported
Figure description
Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear bodies.
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
Antibody dilution
1:10
1:2000
Literature conformity
The subcellular location is supported by literature.
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA030521
Antibody HPA030522
Antibody HPA030523
Antibody CAB000148
Formal validation: Orthogonal
Validated
Spearman correlation >0.6 for protein and RNA expression in tissues.
Validated
Spearman correlation >0.6 for protein and RNA expression in tissues.
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Validated
Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Pearson correlation with HPA030523 across 46 cell lines. Pearson correlation with HPA030522 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA030523 across 46 cell lines. Pearson correlation with HPA030521 across 46 cell lines.
Distribution of protein expression (antibody staining). Pearson correlation with HPA030522 across 46 cell lines. Pearson correlation with HPA030521 across 46 cell lines.
Formal validation: Independent
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Validated
Spearman correlation >0.6 for protein expression in tissues using independent antibodies.
Figure description
Distribution of protein expression (antibody staining). Spearman correlation with HPA030523 across 73 cell types. Spearman correlation with HPA030522 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA030523 across 73 cell types. Spearman correlation with HPA030521 across 73 cell types.
Distribution of protein expression (antibody staining). Spearman correlation with HPA030522 across 73 cell types. Spearman correlation with HPA030521 across 73 cell types.
Standard validation
Supported
Supported
Supported
Supported
Figure description
Immunohistochemical staining of human duodenum shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
Immunohistochemical staining of human bone marrow shows strong nuclear positivity in hematopoietic cells.
Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.
Expression
RNA: detected in 37 tissues Protein: detected in 53 cell types
RNA: detected in 37 tissues Protein: detected in 62 cell types
RNA: detected in 37 tissues Protein: detected in 37 cell types
RNA: detected in 37 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:30
1:175
1:25
1:32000
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
Consistent with RNA expression data.
WESTERN BLOT
Antibody HPA030521
Antibody HPA030522
Antibody HPA030523
Antibody CAB000148
Standard validation
Supported
Analysis performed using a standard panel of samples. Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
28.8
28.8
28.8
28.8
Antibody dilution
1:250
1:250
1:250
1:500
PROTEIN ARRAY
Antibody HPA030521
Antibody HPA030522
Antibody HPA030523
Antibody CAB000148
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
