CNDP2 - Antibodies - The Human Protein Atlas

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CNDP2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA036898

Antibody HPA036899

Antibody CAB026196

Provider

Atlas Antibodies
Sigma-Aldrich

R&D Systems

Product name

HPA036898

HPA036899

MAB3560

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Protein A/G

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA036898

Antibody HPA036899

Antibody CAB026196

Immunocytochemistry

Immunohistochemistry

Western blot

Supported

Protein array

Supported

Approved

IMMUNOCYTOCHEMISTRY

Antibody HPA036898

Antibody HPA036899

Antibody CAB026196

Formal validation: Independent

Validated

Antibody staining overlaps with antibody HPA036899.

Validated

Antibody staining overlaps with antibody HPA036898.

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.

Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm & cytosol.

Antibody dilution

1:109

1:67

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA036898

Antibody HPA036899

Antibody CAB026196

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Formal validation: Independent

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Validated

Pearson correlation >0.6 for protein expression in cell lines using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Pearson correlation with HPA036899 across 46 cell lines.

Distribution of protein expression (antibody staining).
Pearson correlation with HPA036898 across 46 cell lines.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA036899 across 75 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA036898 across 75 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Expression

RNA: detected in 37 tissues
Protein: detected in 62 cell types

RNA: detected in 37 tissues
Protein: detected in 64 cell types

Retrieval

HIER pH6

Antibody dilution

1:3000

1:4500

1:2500

Literature conformity

Consistent with gene/protein characterization data.

RNA consistency

Mainly consistent with RNA expression data.

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA036898

Antibody HPA036899

Antibody CAB026196

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

52.9, 27.7, 17.3, 16.5, 15.2, 14.9, 14.3, 13.7, 13.6, 9.9

52.9, 43.8, 27.7

52.9, 43.8, 27.7, 22.4, 17.3, 16.5, 15.9, 15.2, 14.9, 14.3, 13.7, 13.6, 13.3, 12.5, 9.9, 9.4, 6.4

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA036898

Antibody HPA036899

Antibody CAB026196

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA036898

Antibody HPA036899

Antibody CAB026196

Antigen

Recombinant protein fragment

Recombinant protein

Length (aa)

Antigen sequence

AKWVAIQSVSAWPEKRGEIRRMMEVAAADVKQLGGSVELVDIGKQKLPDG
SEIPLPPILLGRLGSDPQKKTVCI

IPVNVRFCLEGMEESGSEGLDELIFARKDTFFKDVDYVCISDNYWLGKKK
PCITYGLRGICYFFIEVECSNKDLHSGVYGGSVHEAMTDLILLMGS

Matching transcripts

CNDP2-001 - ENSP00000325548 [100%]
CNDP2-004 - ENSP00000462311 [100%]
CNDP2-007 - ENSP00000482227 [100%]
CNDP2-011 - ENSP00000464151 [100%]
CNDP2-014 - ENSP00000463314 [100%]
CNDP2-016 - ENSP00000463702 [100%]
CNDP2-019 - ENSP00000462673 [100%]
CNDP2-021 - ENSP00000463182 [100%]
CNDP2-022 - ENSP00000463222 [100%]
CNDP2-031 - ENSP00000464014 [100%]
CNDP2-017 - ENSP00000463816 [91%]

CNDP2-001 - ENSP00000325548 [100%]
CNDP2-004 - ENSP00000462311 [100%]
CNDP2-006 - ENSP00000325756 [100%]
CNDP2-022 - ENSP00000463222 [100%]

Other gene match

ANTIGEN VIEW

CNDP2-001	CNDP2-004	CNDP2-006	CNDP2-007	CNDP2-011	CNDP2-014	CNDP2-016	CNDP2-017	CNDP2-019	CNDP2-021	CNDP2-022	CNDP2-031

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.