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LOXL2

ANTIBODY INFORMATION
 
 

Antibody HPA036257

  

Antibody HPA056542

  

Antibody CAB025848

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   R&D Systems
  

Product name

 HPA036257   HPA056542   MAB2639  

Host species

 Rabbit   Rabbit   Mouse  

Clonality

 pAb   pAb   mAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Protein A/G  

Other gene match

      

Released in version

 12   12   5  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA036257

  

Antibody HPA056542

  

Antibody CAB025848

  
Immunocytochemistry


Validated
  

Validated
    
Immunohistochemistry

    Approved  
Western blot

Uncertain   Uncertain   Uncertain  
Protein array

Supported   Supported    

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA036257

  

Antibody HPA056542

  

Antibody CAB025848

  

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA056542.		  
Validated

Antibody staining overlaps with antibody HPA036257.		    
 

Standard validation

 Supported   Supported    

     

Figure description

 Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm.    

Antibody dilution

 1:29   1:39    

Literature conformity

 The subcellular location is supported by literature.   The subcellular location is supported by literature.    

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036257

  

Antibody HPA056542

  

Antibody CAB025848

  

Standard validation

     Approved  

     

Figure description

     Immunohistochemical staining of human pancreas shows moderate positivity in exocrine cells.  

Expression

     RNA: detected in 36 tissues
Protein: detected in 55 cell types		  

Retrieval

     HIER pH6  

Antibody dilution

     1:35  

Literature conformity

     Partly consistent with extensive gene/protein characterization data.  

RNA consistency

     Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036257

  

Antibody HPA056542

  

Antibody CAB025848

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Only bands not corresponding to the predicted size.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		   Uncertain

Analysis performed using a standard panel of samples.
No bands detected.		  

     

Figure description

   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 86.7   86.7   86.7, 27.8, 23.8, 22.3, 16.8, 15.5, 11.2, 10.9  

Antibody dilution

 1:250   1:130   1:500  

PROTEIN ARRAY
 
 

Antibody HPA036257

  

Antibody HPA056542

  

Antibody CAB025848

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		    

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.    

Antibody dilution

 1:3000   1:1550    

ANTIGEN INFORMATION
 
 

Antibody HPA036257

  

Antibody HPA056542

  

Antibody CAB025848

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein  

Length (aa)

 70   63    

Antigen sequence

 
CKHTEDVGVVCSDKRIPGFKFDNSLINQIENLNIQVEDIRIRAILSTYRK
RTPVMEGYVEVKEGKTWKQI
  
FGFPGERTYNTKVYKMFASRRKQRYWPFSMDCTGTEAHISSCKLGPQVSL
DPMKNVTCENGLP
  

											 
																				

																		

										
											
Matching transcripts

												
																					LOXL2-001 - ENSP00000373783 [100%] 

											 
																						LOXL2-001 - ENSP00000373783 [100%] 

											 
																						
											 
																				

																		

										
											
Other gene match

												
																					
											 
																						
											 
																						
											 
																				

																		

										ANTIGEN VIEW
									
									
										

		
			

				

										
						

							

								

									
LOXL2-001
								

							

						

							
									

			

				
	

	

		
					
	

													
															
				
						

				 
			

		

			
	











	

		


			

				

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