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Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
Antibody dilution
1:200
Literature conformity
The subcellular location is partly supported by literature or no literature is available.
IMMUNOHISTOCHEMISTRY
Antibody HPA003256
Antibody CAB004999
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells and in islets of Langerhans.
Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.
Expression
RNA: detected in 35 tissues Protein: detected in 74 cell types
RNA: detected in 35 tissues Protein: detected in 57 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:500
1:100
Literature conformity
Consistent with extensive gene/protein characterization data.
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA003256
Antibody CAB004999
Standard validation
Supported
Band of predicted size in kDa (+/-20%) with additional bands present.
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY406904)
Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
