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Immunofluorescent staining of human cell line A-431 shows localization to vesicles.
Antibody dilution
1:10
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA028162
Antibody HPA028652
Standard validation
Approved
Figure description
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity with granular pattern in renal tubules.
Expression
RNA: detected in 37 tissues Protein: detected in 69 cell types
Retrieval
HIER pH6
Antibody dilution
1:15
Literature conformity
Partly consistent with extensive gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA028162
Antibody HPA028652
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Uncertain
No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416538)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416538)
Target mass (kDa)
83.3, 33.6, 29.6
83.3, 64.6
Antibody dilution
1:250
1:250
PROTEIN ARRAY
Antibody HPA028162
Antibody HPA028652
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
