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DOCK2

ANTIBODY INFORMATION
 
 

Antibody HPA036469

  

Provider

 Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA036469  

Host species

 Rabbit  

Clonality

 pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

  

Released in version

 12  

References

  

VALIDATION SUMMARY
 
 

Antibody HPA036469

  
Immunohistochemistry


Validated
  
Western blot

Uncertain  
Protein array

Supported  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA036469

  

Formal validation: Orthogonal
Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.		  

 

 

  

Figure description

 Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.  
 

Standard validation

 Supported  

 

Figure description

 Immunohistochemical staining of human lymph node shows strong cytoplasmic positivity in germinal and non-germinal center cells.  

Expression

 RNA: detected in 35 tissues
Protein: detected in 21 cell types		  

Retrieval

 HIER pH6  

Antibody dilution

 1:300  

Literature conformity

 Consistent with extensive gene/protein characterization data.  

RNA consistency

 Consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA036469

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

 

Figure description

  

Target mass (kDa)

 211.9, 153.4  

Antibody dilution

 1:250  

PROTEIN ARRAY
 
 

Antibody HPA036469

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

 

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA036469

  

Antigen

 Recombinant protein fragment  

Length (aa)

 81  

Antigen sequence

 
SVLSQMSFASQSMPTIPALALSVAGIPGLDEANTSPRLSQTFLQLSDGDK
KTLTRKKVNQFFKTMLASKSAEEGKQIPDSL
 

Matching transcripts

 DOCK2-001 - ENSP00000256935 [100%]
DOCK2-004 - ENSP00000429283 [100%]
 

Other gene match

  
ANTIGEN VIEW
DOCK2-001
DOCK2-004
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.