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ANTIBODY INFORMATION
 
 

Antibody HPA017936

  

Antibody HPA018950

  

Antibody HPA018954

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA017936   HPA018950   HPA018954  

Host species

 Rabbit   Rabbit   Rabbit  

Clonality

 pAb   pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

      

Released in version

 4   5   4  

References

      

VALIDATION SUMMARY
 
 

Antibody HPA017936

  

Antibody HPA018950

  

Antibody HPA018954

  
Immunocytochemistry

  Supported  

Validated
  
Immunohistochemistry

Supported  

Validated
  

Validated
  
Western blot

Supported   Supported  

Validated
  
Protein array

Supported   Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA017936

  

Antibody HPA018950

  

Antibody HPA018954

  

Formal validation: Genetic

    
Validated

Significant downregulation > 25% by both siRNA:s.		  

   
 

Relative fluorescence intensity (RFI)

  

Figure description

     Cytoplasmic region of segmented cells in 10x-images  

Antibody dilution

     1:50  
 

Standard validation

   Supported   Supported  

     

Figure description

   Immunofluorescent staining of human cell line U-251 MG shows localization to nuclear bodies & cytosol.   Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.  

Antibody dilution

   1:40   1:53  

Literature conformity

   The subcellular location is supported by literature.   The subcellular location is supported by literature.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA017936

  

Antibody HPA018950

  

Antibody HPA018954

  

Formal validation: Independent

  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  
Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.		  

 
 

  
 

  

Figure description

   Distribution of protein expression (antibody staining).
Spearman correlation with HPA018954 across 74 cell types.		   Distribution of protein expression (antibody staining).
Spearman correlation with HPA018950 across 74 cell types.		  
 

Standard validation

 Supported   Supported   Supported  

     

Figure description

 Immunohistochemical staining of human kidney shows distinct cytoplasmic and membranous positivity in tubular cells.   Immunohistochemical staining of human hippocampus shows strong cytoplasmic positivity in neuronal cells.   Immunohistochemical staining of human colon shows strong positivity in glandular cells.  

Expression

 RNA: detected in 37 tissues
Protein: detected in 81 cell types		   RNA: detected in 37 tissues
Protein: detected in 53 cell types		   RNA: detected in 37 tissues
Protein: detected in 69 cell types		  

Retrieval

 HIER pH6   HIER pH6   HIER pH6  

Antibody dilution

 1:300   1:175   1:100  

Literature conformity

 Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.   Consistent with extensive gene/protein characterization data.  

RNA consistency

 Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.   Mainly consistent with RNA expression data.  

WESTERN BLOT
 
 

Antibody HPA017936

  

Antibody HPA018950

  

Antibody HPA018954

  

Formal validation: Genetic

    
Validated

Downregulation visible in both siRNA lanes		  

     

Figure description

     Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10
Lane 2: siRNA 1
Lane 3: siRNA 2
Lane 4: Scrambled		  

Target mass (kDa)

     59.1  

Loading control

     Total protein image  

Antibody dilution

     1:117  
 

Standard validation

 Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		   Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).		  

     

Figure description

 Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		   Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil		  

Target mass (kDa)

 59.1, 43.9   59.1   59.1  

Antibody dilution

 1:250   1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA017936

  

Antibody HPA018950

  

Antibody HPA018954

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

     

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:3000   1:3000   1:3000  

ANTIGEN INFORMATION
 
 

Antibody HPA017936

  

Antibody HPA018950

  

Antibody HPA018954

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 118   97   133  

Antigen sequence

 
SKEYTLDVYRLSSVVTQHDSKKAGAEVVKQVEHPLLSGLLYPGLQALDEE
YLKVDAQFGGIDQRKIFTFAEKYLPALGYSKRVHLMNPMVPGLTGSKMSS
SEEESKIDLLDRKEDVKK
  
VLCNLKPQKMRGVESQGMLLCASIEGINRQVEPLDPPAGSAPGEHVFVKG
YEKGQPDEELKPKKKVFEKLQADFKISEECIAQWKQTNFMTKLGSIS
  
GNVENNGVLSFIKHVLFPLKSEFVILRDEKWGGNKTYTAYVDLEKDFAAE
VVHPGDLKNSVEVALNKLLDPIREKFNTPALKKLASAAYPDPSKQKPMAK
GPAKNSEPEEVIPSRLDIRVGKIITVEKHPDAD
 

Matching transcripts

 YARS-001 - ENSP00000362576 [100%]
YARS-201 - ENSP00000484192 [100%]
  YARS-001 - ENSP00000362576 [100%]
  YARS-001 - ENSP00000362576 [100%]
 

Other gene match

      
ANTIGEN VIEW
YARS-001
YARS-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.