DSC2 - Antibodies - The Human Protein Atlas

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DSC2

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA011911

Antibody HPA012615

Provider

Atlas Antibodies
Sigma-Aldrich

Product name

HPA011911

HPA012615

Host species

Rabbit

Clonality

pAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Other gene match

Released in version

References

VALIDATION SUMMARY

Antibody HPA011911

Antibody HPA012615

Immunohistochemistry

Western blot

Supported

Protein array

Supported

IMMUNOHISTOCHEMISTRY

Antibody HPA011911

Antibody HPA012615

Formal validation: Orthogonal

Validated

Pearson correlation >0.6 for protein and RNA expression in cell lines.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 44 cell lines.

Formal validation: Orthogonal

Validated

Spearman correlation >0.6 for protein and RNA expression in tissues.

Figure description

Distribution of protein expression (antibody staining) and RNA expression (TPM) across 37 tissues.

Formal validation: Independent

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Validated

Spearman correlation >0.6 for protein expression in tissues using independent antibodies.

Figure description

Distribution of protein expression (antibody staining).
Spearman correlation with HPA012615 across 73 cell types.

Distribution of protein expression (antibody staining).
Spearman correlation with HPA011911 across 73 cell types.

Standard validation

Supported

Figure description

Immunohistochemical staining of human esophagus shows strong membranous positivity in squamous epithelial cells.

Expression

RNA: detected in 34 tissues
Protein: detected in 33 cell types

RNA: detected in 34 tissues
Protein: detected in 34 cell types

Retrieval

HIER pH6

Antibody dilution

1:200

1:250

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

WESTERN BLOT

Antibody HPA011911

Antibody HPA012615

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

100, 93.8

Antibody dilution

1:250

PROTEIN ARRAY

Antibody HPA011911

Antibody HPA012615

Standard validation

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

ANTIGEN INFORMATION

Antibody HPA011911

Antibody HPA012615

Antigen

Recombinant protein fragment

Length (aa)

140

116

Antigen sequence

IGVVNEAPFSREASPRSAMSTATVTVNVEDQDEGPECNPPIQTVRMKENA
EVGTTSNGYKAYDPETRSSSGIRYKKLTDPTGWVTIDENTGSIKVFRSLD
REAETIKNGIYNITVLASDQGGRTCTGTLGIILQDVNDNS

KTVIICKPTMSSAEIVAVDPDEPIHGPPFDFSLESSTSEVQRMWRLKAIN
DTAARLSYQNDPPFGSYVVPITVRDRLGMSSVTSLDVTLCDCITENDCTH
RVDPRIGGGGVQLGKW

Matching transcripts

DSC2-001 - ENSP00000280904 [100%]
DSC2-002 - ENSP00000251081 [100%]

Other gene match

ANTIGEN VIEW

DSC2-001	DSC2-002

RELEVANT PUBLICATIONS

Antibody HPA011911

Antibody HPA012615

Mechanistic insights into arrhythmogenic right ventricular cardiomyopathy caused by desmocollin-2 mutations
Gehmlich K et al
Cardiovasc Res 2011;90(1):77-87

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.