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Immunohistochemical staining of human colon shows strong cytoplasmic positivity in peripheral nerves and ganglion cells.
Immunohistochemical staining of human lymph node shows moderate cytoplasmic positivity in germinal center cells and non-germinal center cells.
Immunohistochemical staining of human duodenum shows distinct positivity in ganglion cells.
Expression
RNA: detected in 13 tissues Protein: detected in 5 cell types
RNA: detected in 13 tissues Protein: detected in 21 cell types
RNA: detected in 13 tissues Protein: detected in 2 cell types
Retrieval
HIER pH6
HIER pH6
HIER pH6
Antibody dilution
1:150
1:7500
1:50
Literature conformity
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
Consistent with extensive gene/protein characterization data.
RNA consistency
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
No internal RNA expression data available for correlation.
WESTERN BLOT
Antibody HPA039277
Antibody HPA063887
Antibody CAB002437
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band differing more than +/-20% from predicted size in kDa and not supported by experimental and/or bioinformatic data.
Uncertain
Analysis performed using a standard panel of samples. Weak band of predicted size but with additional bands of higher intensity also present.
Uncertain
Analysis performed using a standard panel of samples. No bands detected.
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
53.7
53.7, 22.8
53.7, 24.5, 22.8
Antibody dilution
1:250
1:500
1:500
PROTEIN ARRAY
Antibody HPA039277
Antibody HPA063887
Antibody CAB002437
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
