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Immunofluorescent staining of human cell line SH-SY5Y shows localization to intermediate filaments.
Antibody dilution
1:30
Literature conformity
The subcellular location is not consistent with literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA018790
Antibody HPA048800
Standard validation
Supported
Supported
Figure description
Immunohistochemical staining of human testis shows distinct cytoplasmic positivity in mature germinal cells in seminiferus ducts.
Immunohistochemical staining of human cerebral cortex shows distinct cytoplasmic positivity in neuronal cells and neuropil.
Expression
RNA: detected in 29 tissues Protein: detected in 21 cell types
RNA: detected in 29 tissues Protein: detected in 19 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:125
1:15
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA018790
Antibody HPA048800
Standard validation
Uncertain
Analysis performed using a standard panel of samples. Single band larger than predicted size in kDa (+20%) but partly supported by experimental and/or bioinformatic data.
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY415867)
Target mass (kDa)
35.1, 27.3
35.1, 30.4, 27.3, 12.5
Antibody dilution
1:250
1:125
PROTEIN ARRAY
Antibody HPA018790
Antibody HPA048800
Standard validation
Approved
Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:1000
ANTIGEN INFORMATION
Antibody HPA018790
Antibody HPA048800
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
45
51
Antigen sequence
MTQGKLSVANKAPGTEGQQQVHGEKKEAPAVPSAPPSYEEATSGE
PTAVPLHPSWAYVDPSSSSSYDNGFPTGDHELFTTFSWDDQKVRRVFVRK
V
