HNRNPA1 - Antibodies - The Human Protein Atlas

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HNRNPA1

TISSUE

CELL

CANCER

ANTIBODY INFORMATION

Antibody HPA001609

Antibody HPA001666

Antibody HPA005812

Antibody HPA007185

Antibody CAB010894

Provider

Atlas Antibodies
Sigma-Aldrich

SIGMA

Product name

HPA001609

HPA001666

HPA005812

HPA007185

R4528

Host species

Rabbit

Mouse

Clonality

pAb

mAb

Purity

Affinity purified using the PrEST-antigen as affinity ligand

Not known

Other gene match

HNRNPA2B1 - ENSG00000122566 [100%]
HNRNPA3 - ENSG00000170144 [81%]

Released in version

References

VALIDATION SUMMARY

Antibody HPA001609

Antibody HPA001666

Antibody HPA005812

Antibody HPA007185

Antibody CAB010894

Immunocytochemistry

Immunohistochemistry

Western blot

Protein array

IMMUNOCYTOCHEMISTRY

Antibody HPA001609

Antibody HPA001666

Antibody HPA005812

Antibody HPA007185

Antibody CAB010894

Standard validation

Supported

Figure description

Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm.

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

Antibody dilution

1:25

1:4

Literature conformity

The subcellular location is supported by literature.

IMMUNOHISTOCHEMISTRY

Antibody HPA001609

Antibody HPA001666

Antibody HPA005812

Antibody HPA007185

Antibody CAB010894

Standard validation

Supported

Figure description

Immunohistochemical staining of human fallopian tube shows strong nuclear positivity.

Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.

Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.

Expression

RNA: detected in 37 tissues
Protein: detected in 78 cell types

RNA: detected in 37 tissues
Protein: detected in 72 cell types

RNA: detected in 37 tissues
Protein: detected in 77 cell types

Retrieval

HIER pH6

Antibody dilution

1:400

1:25

1:600

Literature conformity

Consistent with extensive gene/protein characterization data.

RNA consistency

Consistent with RNA expression data.

Mainly consistent with RNA expression data.

WESTERN BLOT

Antibody HPA001609

Antibody HPA001666

Antibody HPA005812

Antibody HPA007185

Antibody CAB010894

Standard validation

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Supported

Analysis performed using a standard panel of samples.
Single band corresponding to the predicted size in kDa (+/-20%).

Supported

Analysis performed using a standard panel of samples.
Band of predicted size in kDa (+/-20%) with additional bands present.

Figure description

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 16.8
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17
Lane 2: RT4
Lane 3: U-251 MG
Lane 4: Human Plasma
Lane 5: Liver
Lane 6: Tonsil

Target mass (kDa)

38.7, 34.2, 33.2, 29.4, 25.7

39.6, 38.7, 37.4, 37, 36, 34.2, 33.2, 29.8, 29.4, 25.7

38.7

38.7, 34.2, 33.2, 29.4, 25.7, 19.5, 16.8, 16.5, 12.9

Antibody dilution

1:250

1:500

PROTEIN ARRAY

Antibody HPA001609

Antibody HPA001666

Antibody HPA005812

Antibody HPA007185

Antibody CAB010894

Standard validation

Approved

Pass with quality comment low specificity (binding to 1-2 PrESTs >15% and <40%).

Supported

Pass with single peak corresponding to interaction only with its own antigen.

Figure description

Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.

Antibody dilution

1:3000

1:500

1:3000

1:500

ANTIGEN INFORMATION

Antibody HPA001609

Antibody HPA001666

Antibody HPA005812

Antibody HPA007185

Antibody CAB010894

Antigen

Recombinant protein fragment

Not known

Length (aa)

126

137

Antigen sequence

ETTDESLRSHFERWGMLTDCAVMRDPNTKRSRGFGFVTYATVEEVDAATN
ARPHKVDGKVVEPRRTVSREDYQRSGAHLTVKKIFVGGIKENTEKHQLRD
YFEQHGKMEVIEIMTEAVARKGALPL

VMRDPASKRSRGFGFVTFSSMAEVDAAMAARPHSIDGRVVEPKRAVAREE
SGKPGAHVTVKKLFVGGIKEDTEEHHLRDYFEEYGKIDTIEIITDRQSGK
KRGFGFVTFDDHDPVDKIVLQKYHTINGHNAEVRKAL

VMRDPASKRSRGFGFVTFSSMAEVDAAMAARPHSIDGRVVEPKRAVAREE
SGKPGAHVTVKKLFVGGIKEDTEEHHLRDYFEEYGKIDTIEIITDRQSGK
KRGFGFVTFDDHDPVDKIVLQKYHTINGHNAEVRKAL

GGRGGGFGGNDNFGRGGNFSGRGGFGGSRGGGGYGGSGDGYNGFGNDGGY
GGGGPGYSGGSRGYGSGGQGYGNQGSGYGGSGSYDS

Matching transcripts

HNRNPA1-001 - ENSP00000448617 [80%]
HNRNPA1-002 - ENSP00000341826 [80%]
HNRNPA1-012 - ENSP00000447260 [80%]
HNRNPA1-013 - ENSP00000447782 [80%]
HNRNPA1-023 - ENSP00000333504 [80%]

HNRNPA1-001 - ENSP00000448617 [81%]
HNRNPA1-002 - ENSP00000341826 [81%]
HNRNPA1-012 - ENSP00000447260 [81%]
HNRNPA1-013 - ENSP00000447782 [81%]
HNRNPA1-023 - ENSP00000333504 [81%]

HNRNPA1-002 - ENSP00000341826 [100%]

Other gene match

HNRNPA2B1 - ENSG00000122566 [100%]
HNRNPA3 - ENSG00000170144 [81%]

ANTIGEN VIEW

HNRNPA1-001	HNRNPA1-002	HNRNPA1-012	HNRNPA1-013	HNRNPA1-023

Contact

contact@proteinatlas.org

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.