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RNASEH2B

ANTIBODY INFORMATION
 
 

Antibody HPA040084

  

Antibody HPA041469

  

Provider

 Atlas Antibodies
Sigma-Aldrich
   Atlas Antibodies
Sigma-Aldrich
  

Product name

 HPA040084   HPA041469  

Host species

 Rabbit   Rabbit  

Clonality

 pAb   pAb  

Purity

 Affinity purified using the PrEST-antigen as affinity ligand   Affinity purified using the PrEST-antigen as affinity ligand  

Other gene match

    

Released in version

 7   12  

References

    

VALIDATION SUMMARY
 
 

Antibody HPA040084

  

Antibody HPA041469

  
Immunocytochemistry


Validated

Validated
  

Validated

Validated
  
Immunohistochemistry

Approved    
Western blot

Uncertain   Uncertain  
Protein array

Supported   Supported  

IMMUNOCYTOCHEMISTRY
 
 

Antibody HPA040084

  

Antibody HPA041469

  

Formal validation: Tagged protein
Validated

Antibody staining overlaps with GFP tagged protein		  
Validated

Antibody staining overlaps with GFP tagged protein		  

   

Figure description

 Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus and GFP expression in nucleus.   Immunofluorescent staining of transgenic HeLa cells show antibody staining in nucleus and GFP expression in nucleus.  

Antibody dilution

 1:127   1:4  
 

Formal validation: Independent
Validated

Antibody staining overlaps with antibody HPA041469.		  
Validated

Antibody staining overlaps with antibody HPA040084.		  
 

Standard validation

 Approved   Approved  

   

Figure description

 Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.   Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.  

Antibody dilution

 1:127   1:4  

Literature conformity

 The subcellular location is partly supported by literature or no literature is available.   The subcellular location is partly supported by literature or no literature is available.  

IMMUNOHISTOCHEMISTRY
 
 

Antibody HPA040084

  

Antibody HPA041469

  

Standard validation

 Approved    

   

Figure description

 Immunohistochemical staining of human stomach shows strong nuclear and cytoplasmic positivity in glandular cells.    

Expression

 RNA: detected in 37 tissues
Protein: detected in 60 cell types		    

Retrieval

 HIER pH6    

Antibody dilution

 1:100    

Literature conformity

 Consistent with gene/protein characterization data.    

RNA consistency

 Mainly consistent with RNA expression data.    

WESTERN BLOT
 
 

Antibody HPA040084

  

Antibody HPA041469

  

Standard validation

 Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		   Uncertain

Analysis performed using a standard panel of samples.
Weak band of predicted size but with additional bands of higher intensity also present.		  

   

Figure description

    

Target mass (kDa)

 37.5, 35.1, 29   37.5, 35.1  

Antibody dilution

 1:250   1:250  

PROTEIN ARRAY
 
 

Antibody HPA040084

  

Antibody HPA041469

  

Standard validation

 Supported

Pass with single peak corresponding to interaction only with its own antigen.		   Supported

Pass with single peak corresponding to interaction only with its own antigen.		  

   

Figure description

 Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.   Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.  

Antibody dilution

 1:6000   1:500  

ANTIGEN INFORMATION
 
 

Antibody HPA040084

  

Antibody HPA041469

  

Antigen

 Recombinant protein fragment   Recombinant protein fragment  

Length (aa)

 97   84  

Antigen sequence

 
VVDNVFPNCILLLKLPGLEKLLHHVTEEKGNPEIDNKKYYKYSKEKTLKW
LEKKVNQTVAALKTNNVNVSSRVQSTAFFSGDQASTDKEEDYIRYAH
  
ISDYIPKELSDDLSKYLKLPEPSASLPNPPSKKIKLSDEPVEAKEDYTKF
NTKDLKTEKKNSKMTAAQKALAKVDKSGMKSIDT
 

Matching transcripts

 RNASEH2B-001 - ENSP00000337623 [100%]
RNASEH2B-009 - ENSP00000389877 [100%]
RNASEH2B-201 - ENSP00000481236 [100%]
  RNASEH2B-001 - ENSP00000337623 [100%]
RNASEH2B-201 - ENSP00000481236 [100%]
 

Other gene match

    
ANTIGEN VIEW
RNASEH2B-001
RNASEH2B-009
RNASEH2B-201
 

Contact

The Project

The Human Protein Atlas

The Human Protein Atlas project is funded
by the Knut & Alice Wallenberg foundation.