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Immunofluorescent staining of human cell line MCF7 shows localization to cytosol.
Antibody dilution
1:27
Literature conformity
The subcellular location is supported by literature.
IMMUNOHISTOCHEMISTRY
Antibody HPA008067
Antibody HPA054177
Standard validation
Approved
Approved
Figure description
Immunohistochemical staining of human liver shows strong nuclear and cytoplasmic positivity in hepatocytes.
Immunohistochemical staining of human liver shows moderate cytoplasmic positivity in hepatocytes.
Expression
RNA: detected in 37 tissues Protein: detected in 58 cell types
RNA: detected in 37 tissues Protein: detected in 49 cell types
Retrieval
HIER pH6
HIER pH6
Antibody dilution
1:50
1:35
Literature conformity
Consistent with gene/protein characterization data.
Consistent with gene/protein characterization data.
RNA consistency
Mainly consistent with RNA expression data.
Mainly consistent with RNA expression data.
WESTERN BLOT
Antibody HPA008067
Antibody HPA054177
Standard validation
Supported
Single band corresponding to the predicted size in kDa (+/-20%).
Supported
Analysis performed using a standard panel of samples. Single band corresponding to the predicted size in kDa (+/-20%).
Figure description
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: Negative control (vector only transfected HEK293T lysate) Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY416629)
Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 Lane 2: RT4 Lane 3: U-251 MG Lane 4: Human Plasma Lane 5: Liver Lane 6: Tonsil
Target mass (kDa)
26.1, 23.3, 20.5
23.3
Antibody dilution
1:250
1:140
PROTEIN ARRAY
Antibody HPA008067
Antibody HPA054177
Standard validation
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Supported
Pass with single peak corresponding to interaction only with its own antigen.
Figure description
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody specificity analysis with protein arrays. Predicted and matching interactions are shown in green.
Antibody dilution
1:3000
1:1100
ANTIGEN INFORMATION
Antibody HPA008067
Antibody HPA054177
Antigen
Recombinant protein fragment
Recombinant protein fragment
Length (aa)
101
101
Antigen sequence
VLPKTSWNIPQPGEGDVREEALSTGGLDLIFMPGLGFDKHGNRLGRGKGY
YDAYLKRCLQHQEVKPYTLALAFKEQICLQVPVNENDMKVDEVLYEDSST
A
AAAAVSSAKRSLRGELKQRLRAMSAEERLRQSRVLSQKVIAHSEYQKSKR
ISIFLSMQDEIETEEIIKDIFQRGKICFIPRYRFQSNHMDMVRIESPEEI
S
